Dual Media SetAlthough recombinant protein expression in E. coli has become all but routine, high level protein expression or over expression is not always consistent and repeatable for every protein. Our research at Zymo Research Corp. has shown that high level protein expression can be achieved consistently when two processes cell expansion and protein expression are completely separated. The Dual Media Set, different from commonly used protein expression

in ≥ 25 µl eluate

bisulfite conversion/methylation analysis

chemically inert

ZymoTaq qPCR PreMix contains all the reagents needed to perform quantitative PCR and other molecular downstream analysis such as high-resolution melt (HRM) analysis of DNA methylation and other general real-time PCR assays

Component for sealing the EZ-Vac Vacuum Manifold (Cat

The procedure is easy

The DNA Denaturing Buffer is designed to facilitate the denaturation of DNA for the subsequent methylated DNA immunoprecipitation in the Methylated-DNA IP Kit

Human DNA can be reliably quantified in a background of non-human DNA

DNA Pre-Wash Buffer is designed for the removal of protein contaminants

Sequencing Binding Buffer is used in the ZR Sequencing Clean-up Kits for the efficient binding of DNA from post-cycle sequencing reactions and subsequent rapid removal of reaction contaminants (i

and re-suspension steps common to conventional procedures

sediment and/or fecal samples